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Use of solid-phase PCR for enhanced detection of airborne microorganisms.
Authors:A J Alvarez   M P Buttner   G A Toranzos   E A Dvorsky   A Toro   T B Heikes   L E Mertikas-Pifer     L D Stetzenbach
Abstract:The solid-phase PCR (SP-PCR) was compared with a culture-based technique for the detection of aerosolized Escherichia coli DH1. Results with SP-PCR showed an increase in detection sensitivity over that of culture methods. Therefore, SP-PCR may be useful for the detection of airborne microorganisms which may be nonculturable because of aerosolization or sampling stress.
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