Binding of puromycin to E. coli ribosomes. Effects of puromycin analogues and peptide bond formation inhibitors |
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Authors: | Rafael Fernandez-Muñoz David Vazquez |
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Affiliation: | (1) Instituto de Biología Celular, Madrid, Spain |
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Abstract: | 3H-puromycin binds to bacterial ribosomes, in the presence of ethanol, under the experimental conditions of the fragment reaction assay. The binding is feeble, takes place at 0°C, is partially inhibited by chloramphenicol and lincomycin and totally by sparsomycin. 3H-puromycin binding is hardly affected by the 3 aminonucleoside of puromycin, is well inhibited by the L-Phenylalanine and L-Leucine analogues of puromycin and totally blocked by L-Phenylalanyl-adenosine. |
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