Neutral endopeptidase-24.11 (NEP) deactivates PDGF- and TGF-beta-induced cell shape changes in invertebrate immunocytes |
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Authors: | Caselgrandi E Kletsas D Ottaviani E |
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Affiliation: | Department of Biomedical Sciences, Hygiene and Microbiology Section, University of Modena and Reggio Emilia, Modena, 41100, Italy. |
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Abstract: | Using a cytofluorimetric assay, we found that immunocytes of the mollusc, Mytilus galloprovincialis, express CD10, a surface antigen known to be identical to neutral endopeptidase-24.11 (NEP). The spectrofluorimetric analysis demonstrates that the growth factors PDGF-AB and TGF-beta1 provoke an increase in NEP-like activity in membrane preparations from the immunocytes, but have no effect on the soluble form in the serum. On the other hand, computer-assisted microscopic image analysis reveals that NEP deactivates the PDGF-AB- and TGF-beta1-induced shape changes in immunocytes. However, Western blots show that, in solution, NEP does not cleave PDGF-AB or TGF-beta1, indicating that the inactivation is not due to proteolysis. These results suggest a functional interplay in invertebrate immunocytes between growth factors and NEP, as previously shown in vertebrate cells. |
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Keywords: | molluscan immunocytes neutral endopeptidase‐24.11 (NEP) platelet‐derived growth factor (PDGF)‐AB transforming growth factor (TGF)‐β1 CD10 cell shape changes |
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