| 人红细胞带3蛋白胞质段的克隆及其表达 |
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| 引用本文： | 李至,彭锋,季朝能,宦春雷,张志鸿.人红细胞带3蛋白胞质段的克隆及其表达[J].生物化学与生物物理进展,1999,26(5):492-494. |
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| 作者姓名： | 李至 彭锋 季朝能 宦春雷 张志鸿 |
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| 作者单位： | 复旦大学生命科学学院,立人实验室,上海,200433 |
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| 基金项目： | 国家自然科学基金资助项目（39730150，39570203）. |
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| 摘 要： | 人红细胞带3蛋白胞质段(cytoplasmic domain of band 3, cdb3)起着将膜与膜骨架、细胞内环境相联系的重要作用. 以带3蛋白全长基因为模板，用PCR方法扩增出cdb3片段，克隆至pRSET表达质粒上，转化大肠杆菌BL21(DE3). 转化菌经诱导表达出较高含量的cdb3蛋白，纯化后，测得对醛缩酶有抑制活性.
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| 关 键 词： | 带3蛋白 胞质段，质粒，表达 |
| 收稿时间： | 1998/9/11 0:00:00 |
| 修稿时间： | 1/7/1999 12:00:00 AM |
Cloning and Expression of the Cytoplasmic Domain of Human Erythrocyte Band 3 Protein |
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| LI Zhi,PENG Feng,JI Chao-Neng,HUAN Chun-Lei and ZHANG Zhi-Hong.Cloning and Expression of the Cytoplasmic Domain of Human Erythrocyte Band 3 Protein[J].Progress In Biochemistry and Biophysics,1999,26(5):492-494. |
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| Authors: | LI Zhi PENG Feng JI Chao-Neng HUAN Chun-Lei and ZHANG Zhi-Hong |
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| Institution: | School of Life Sciences, Fudan University, Li Ren Laboratory, Shanghai 200433, China;School of Life Sciences, Fudan University, Li Ren Laboratory, Shanghai 200433, China;School of Life Sciences, Fudan University, Li Ren Laboratory, Shanghai 200433, China;School of Life Sciences, Fudan University, Li Ren Laboratory, Shanghai 200433, China;School of Life Sciences, Fudan University, Li Ren Laboratory, Shanghai 200433, China |
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| Abstract: | The cytoplasmic domain of human erythrocyte band 3 (cdb3) plays an important role in connection of the membrane with the membrane skeleton, and environment inside the cell as well. With whole length of band 3 gene, cdb3 gene was amplified by PCR and then was cloned into pRSET plasmid. The plasmid was introduced into E.coli BL21(DE3). The cdb3 expression proteins were purified and its function was verified according to the ability to inhibit aldolase. |
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| Keywords: | band 3 protein cytoplasmic domain plasmid expression |
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