| 转MDV特异性磷蛋白基因兔的获得及遗传 |
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| 引用本文： | 成国祥,崔治中.转MDV特异性磷蛋白基因兔的获得及遗传[J].生物技术,1994,4(4):7-10. |
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| 作者姓名： | 成国祥 崔治中 |
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| 作者单位： | 江苏农学院农业生物技术研究所 |
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| 摘 要： | 显微注射ＭＤＶ特异性磷蛋白基因进入２１枚受精卵原核，移入受体，共获８只仔兔，２只很快死亡，６只发育到性成熟，用非同位素Ｄｉｇｏｘｉｇｅｕｉｎ标记核酸探针，经斑点杂交证实３只性成熟兔整合注射基因。２只当代转基因兔分别与正常免回交，所产后代，经同样的方法证实都获得第一代转基因兔，这表明ＭＤｖ特异性磷蛋白基因已经整合到它们的生殖细胞系。
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| 关 键 词： | 转基因兔 MDV 磷蛋白基因 遗传 |
The Production and Germ-line Transmission of Transgenic Rabbits with Marek's Disease Virus Gene Encoding Virus-Specific Phosphorylated Polypeptides |
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| Cheng Guoxiang, Cui Zhizhong, Du Fuliang,Cheng Yong, Wang Handong, Xu Shaofu.The Production and Germ-line Transmission of Transgenic Rabbits with Marek's Disease Virus Gene Encoding Virus-Specific Phosphorylated Polypeptides[J].Biotechnology,1994,4(4):7-10. |
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| Authors: | Cheng Guoxiang Cui Zhizhong Du Fuliang Cheng Yong Wang Handong Xu Shaofu |
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| Abstract: | Marek's Disease virus Gene Encoding Virus-Specific Phosphorylated Polypeptides (MDVSpp gene) was microinjected into the pronuclei of 21 eggs in vivo fertilization. 8 offsprings were produced. Two of these died quickly after they were born. Six of these developed to grown-up ones. It is confirmed by dot blot that the gene has been integrated into three grown-up rabbits (DNA Probe labeled with digoxigenin-dUTP). Transgnic Does B3 and B4 were bred with wild--type rabbits. Some of the resulting progeny were integrated the transgene. The experiment results show that the transgene has been integrated in the germ-line of transgnic Does B3 and B4. |
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| Keywords: | MDV Phosphorylated polypeptides gene Gene transfer integration Transmission |
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