| 利用鳍条提取样品总DNA初探 |
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| 引用本文： | 梁利群 孙孝文. 利用鳍条提取样品总DNA初探[J]. 生物技术, 1994, 4(1): 45-46,18 |
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| 作者姓名： | 梁利群 孙孝文 |
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| 作者单位： | 中国水产科学研究院黑龙江水产研究所 |
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| 摘 要： | 本文报告了从鱼类鳍条中抽提基因组ＤＮＡ的方法，并对所得ＤＮＡ样品做了电泳、紫外分光光度分析，ＳｏｕｔｈｅｒｎＢｌｏｔ等分子杂交实验，均证明这个方法是简便的取样方法，又能完整地保存实验鱼类。
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| 关 键 词： | 鳍条 基因组 转基因鱼 DNA 鱼纳 |
Isolation of Genomic DNA from Fish Fin |
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| Liang Liqun, Sun Xiaowen,Wang Peng, Yan Xueqing,Shen Junbao. Isolation of Genomic DNA from Fish Fin[J]. Biotechnology, 1994, 4(1): 45-46,18 |
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| Authors: | Liang Liqun Sun Xiaowen Wang Peng Yan Xueqing Shen Junbao |
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| Affiliation: | Heilongjiang River Fishery Ressearch Institute.Chinese Academy of Fishery Science.Harbin |
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| Abstract: | The method of isolation of genomic DNA from fish fin was reported. Those DNA sample were analoysed by several experiments including electrophotesis ultraviolet spectrophotometric determination and Southern Hybridization. It was proved that this method was simple for performance and Preerved experimental fishes very well. |
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| Keywords: | Fish fin Genomic DNA Transgenic fish. |
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