| 黄瓜离体子叶切块培养直接分化花芽 |
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| 引用本文： | 庞基良,梁海曼.黄瓜离体子叶切块培养直接分化花芽[J].生物技术,1994,4(6):9-11. |
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| 作者姓名： | 庞基良 梁海曼 |
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| 作者单位： | 杭州市农业科学研究所，杭州大学生物系 |
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| 基金项目： | 国家自然科学基金，浙江省自然科学基金 |
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| 摘 要： | ４８小时龄黄瓜幼苗的子叶切块接种于附加２．０ｍｇ／ＬＢＡ及１．０ｍｇ／ＬＡｇＮＯ３的修改ＭＳ培养基上，培养约６０天后在子叶切块的近轴端可直接形成雄花芽，其频率达７．７％。
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| 关 键 词： | 黄瓜 子叶切块培养 花芽分化 硝酸根 |
The Study on Direct Formation of the Floral Buds from Excised Cotyledonary Explants of Cucumis Sativus L. |
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| Pang Jiliang,Sun Jianhong.The Study on Direct Formation of the Floral Buds from Excised Cotyledonary Explants of Cucumis Sativus L.[J].Biotechnology,1994,4(6):9-11. |
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| Authors: | Pang Jiliang Sun Jianhong |
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| Abstract: | Cotyledonary explants from 48-hour-old seedlings of cucumis sativus L. inoculated on modified Murashinge and Skoog (MS) medium supplemented with 2. 0 mg/L benzyladenine (BA) and 1. 0mg/L AgNO3 for about 60 days, directly differentiated male flowers at the adageal end of cotyledonary explants.The percentage of direct formation of floral buds was 7. 7%. If the frequency of flower formation in cucumber cotyledonary explants can be greatly increased, this experimental system can be applied in studying the transition from vegetative growth to reproductive growth and sex expression. |
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| Keywords: | Cucumis sativus L culture of cotyledonary explants Floral differentiation AgNO_3 |
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