| 人铜锌超氧化物歧化酶的大规模生产工艺 |
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| 引用本文： | 吕星 周潮. 人铜锌超氧化物歧化酶的大规模生产工艺[J]. 生物技术, 1994, 4(6): 34-36,27 |
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| 作者姓名： | 吕星 周潮 |
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| 作者单位： | 军事医学科学院放射医学研究所，成都蜀阳制药厂 |
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| 摘 要： | 本文以干扰素生产中废弃的人红细胞为原料，采用乙醇－氯份沉淀→磷酸氢二钾盐析→丙酮沉淀手续和ＤＥＡＥ—５２层析步骤，进行了人铜锌超氧化物歧化酶（ＣｕＺｎ－ＳＯＤ）大规模生产工艺的实验研究。结果表明，生产的酶制剂中含量为９０．６％，比活性在２０００ＭｃＣｏｒｄ—Ｆｒｉｄｏｖｉｃｈ单位／ｍｇ蛋白以上，无菌、无热原质、安全性等项指标符合国家卫生部对血液制品的要求。
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| 关 键 词： | 人铜锌 超氧化物歧化酶 人红细胞 生产工艺 |
Large Scale Production of Human Erythrocyte Copper Zine Superoxide Dismutase |
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| Lu Xing,Chen Jizhong,Sui Jianli,Fang Yunzhong. Large Scale Production of Human Erythrocyte Copper Zine Superoxide Dismutase[J]. Biotechnology, 1994, 4(6): 34-36,27 |
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| Authors: | Lu Xing Chen Jizhong Sui Jianli Fang Yunzhong |
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| Abstract: | A scale up study for the production of copper zinc superoxide dismutase (CuZn-SOD) from discarded human erythrocytes is described in the present paper. The isolation of CuZn-SOD fromhemolysate was accomplished by ethanol- chloroform precipitation, K2HPO4 extraction and acetoneprecipitation. The obtained extracts were futher purified by EDAE-52 chromatography. It is shownthat as many as eightynine percent of protein in the purified material is CuZn-SOD, and the specificactivity is over 2000 McCord -Fridovich unit per mg protein. The human CuZn-SOD Perparationfulfil the requriements of no bacteria contamination, pyrogen-free and safety for the blood productsdesigned by the Ministry of Health. |
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| Keywords: | Human copper zinc superoxide dismutase Human erythrocyte Large scale production |
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