Is the lectin binding pattern of human breast and colon cancer cells influenced by modulators of sialic acid metabolism? |
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Authors: | Udo Schumacher Dhia Mukhtar Peer Stehling Werner Reutter |
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Institution: | 1. Human Morphology, University of Southampton, Bassett Crescent East, SO16 7PX, Southampton, UK 2. Institut für Molekularbiologie und Biochemie, Freie Universit?t Berlin, Arminallee 22, D-14195, Berlin, Germany
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Abstract: | Sialic acid residues are the most abundant terminal carbohydrate residues of mammalian cells. Modification of the sialic acid
residues by exposure of cells in culture to sialic acid precursor analogues resulted in a modifed susceptibility to polyoma
viruses. In the present study, human breast and colon cancer cell lines were exposed for 65 h to these acid precursor analogues
at 5 mM and their lectin binding pattern was analysed. Use of a panel of several different lectins indicated that the pretreatment
of these cell lines with the sialic acid analogues did not change their lectin binding profile. The incorporation of these
precursors into membrane glycoproteins was assessed by reversed phase high-performance liquid chromatography, which clearly
demonstrated that the precursors were incorporated. The results therefore indicate that these analogues are highly specific
for sialic acid and do not interfere with other biosynthetic pathways of membrane glycoconjugates. |
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Keywords: | |
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