Isolation and Partial Characterization of DNA Topoisomerase I from the Nucleoids of White Mustard Chloroplasts |
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| Authors: | Belkina G G Pogul&#;skaya E V Yurina N P |
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| Institution: | (1) Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 119071, Russia |
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| Abstract: | DNA topoisomerase was isolated for the first time from nucleoids of white mustard (Sinapis alba L.) chloroplasts. The enzyme had a molecular weight of 70 kDa; was ATP-independent, required the presence of mono- (K+) and bivalent (Mg2+) cations, and was capable of relaxing both negatively and positively supercoiled DNA. These results suggest that the enzyme isolated belongs to the type IB DNA topoisomerases. |
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