Methanol as the Primary Methanogenic and Acetogenic Precursor in the Cold Zoige Wetland at Tibetan Plateau |
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Authors: | Na Jiang Yanfen Wang Xiuzhu Dong |
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Institution: | (1) Graduate School, Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China;(2) State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No 1, West Beichen Road, Chaoyang District, Beijing, 100101, People’s Republic of China; |
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Abstract: | Previous studies suggested that methanol and acetate were the likely methanogenic precursors in the cold Zoige wetland. In
this study, the contribution of the two substances to methanogenesis and the conversion in Zoige wetland were analyzed. It
was determined that methanol supported the highest CH4 formation rate in the enrichments of the soil grown with Eleocharis valleculosa, and even higher at 15°C than at 30°C; while hydrogenotrophic methanogenesis was higher at 30°C. Both methanol- and acetate-using
methanogens were counted at the highest (107 g−1) in the soil, whereas methanol-using acetogens (108 g−1) were ten times more abundant than either methanol- or acetate-using methanogens. Both methanol and acetate were detected
in the methanogenesis-inhibited soil samples, so that both could be the primary methanogenic precursors in E. valleculosa soil. However, the levels of methanol and acetate accumulated in 2-bromoethane-sulfonate (BES)- and CHCl3-treated soils were in reverse, i.e., higher methanol in CHCl3- and higher acetate in BES-treated soil, so that methanol-derived methanogenesis could be underestimated due to the consumption
by acetogens. Analysis of the soil 16S rRNA genes revealed Acetobacterum bakii and Trichococcus pasteurii to be the dominant methanol-using acetogens in the soil, and a strain of T. pasteurii was isolated, which showed the high conversion of methanol to acetate at 15°C. |
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