Effects of culture conditions on the synthesis of human chorionic gonadotropin by placental organ cultures |
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Authors: | Rachel I. Huot Jean-Michel Foidart Kurt Stromberg |
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Affiliation: | (1) Laboratory of Viral Carcinogenesis, National Cancer Institute, National Institutes of Health, Building 41, Suite 300, 20205 Bethesda, Maryland;(2) Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health, 20205 Bethesda, Maryland;(3) Department of Biology, Catholic University of America, 20064 Washington, D.C. |
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Abstract: | Summary Culture conditions for maintaining first trimester human placenta in organ culture, which enhance the secretion of human chorionic gonadotropin (hCG), are described. Nutrient medium, oxygen tension and Gelfoam support matrix infuence the synthesis of hCG by these cultures. Placental tissue remained viable for the duration of experiments (12 days) as judged by the incorporation of tritiated thymidine into DNA and the lack of release of incorporated [125Iiododeoxyuridine. Optimal conditions for hCG synthesis in placental organ culture included an atmosphere of 95% air and 5% CO2 (approximately 20% O2), CMRL 1066 medium containing fetal human or bovine serum, insulin, hydrocortisone and retinal acetate. Multiple pieces of placenta could be cultured in the same dish with an additive effect on hCG secretion. The functional responsiveness of these placental cultures was demonstrated by modulation of hCG synthesis with theophylline and 3′5′ dibutyryl cyclic AMP. Presented in part at the meeting of the American Association for Cancer Research, April 1978. This work is being submitted in partial fulfillment of the Ph.D. requirements in the Department of Biology, Catholic University of America. |
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Keywords: | human placenta organ culture chorionic gonadotropin |
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