The incorporation of monomethylethanolamine and dimethylethanolamine in fetal brain aggregating cell culture |
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Authors: | Francine Dainous Julian N Kanfer |
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Institution: | (1) Department of Biochemistry, Faculty of Medicine, University of Manitoba, 770 Bannatyne Avenue, R3E 0W3 Winnipeg, Canada |
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Abstract: | Fetal rat brain aggregating cell cultures were exposed to varying concentrations of 3H]monomethylethanolamine (MME) and 3H] dimethylethanolamine (DME). The rate of labeling of water-soluble compounds was more rapid and the amount of radioactivity present was greater than in the lipids. After a 72 hour incubation in the presence of millimolar concentrations of these nitrogenous bases, the major water-soluble products were the phosphorylated form of the bases. Little label was associated with the free bases or their cytidyl derivate. In the phospholipids, 97% of the radioactivity was recovered in phosphatidylmonomethylethanolamine (PMME) and 3% in phosphatidyldimethylethanolamine (PDME) or 95% in PDME and 5% in phosphatidylcholine (PC) after growth in presence of 3H]MME and 3H]DME respectively. The rate of formation of the radioactive products increased as function of the concentration of the nitrogenous base added up to 4 mM, the highest concentration employed. There was no significant difference in the pattern of labeling with cells grown in media devoid of methionine or choline. The turnover of the water-soluble metabolites was more rapid than in the phospholipids where an apparent half-life of 24 hours was calculated.Abbreviations PMT
phospholipid-N-methyltransferase
- AdoMet
S-adenosyl-L-methionine
- EA
ethanolamine
- MME
N-monomethylethanolamine
- DME
N,N-dimethylethanolamine
- CH
choline
- PE
phosphatidylethanolamine
- PMME
phosphatidylmonomethylethanolamine
- PDME
phosphatidyldimethylethanolamine
- PC
phosphatidylcholine
- PS
phosphatidylserine
- CAPS
cyclohexylaminopropane sulfonic acid |
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Keywords: | Aggregating nerve cell culture monomethylethanolamine dimethylethanolamine phospholipid-N-methyltransferase |
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