Stem cell expression and development of trunk musculature of lesser‐spotted dogfish (Scyliorhinus canicula) reveal differences between sharks and teleosts |
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| Authors: | Bianka Grunow Tina Kirchhoff Timo Moritz |
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| Institution: | 1. Zoological Institute and Museum, Cytology and Evolutionary Biology, Ernst‐Moritz‐Arndt‐University of Greifswald, Greifswald, Germany;2. Deutsches Meeresmuseum, Stralsund, Germany;3. Institute of Systematic Zoology and Evolutionary Biology, Friedrich‐Schiller‐University Jena, Jena, Germany |
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| Abstract: | This study compares the trunk skeletal muscle anatomy in 870‐ and 2900‐degree‐day‐old lesser‐spotted dogfish larvae (Scyliorhinus canicula) via haematoxylin/eosin staining as well as immunohistochemistry and Western blot analysis. The results showed poorly differentiated muscle formation in the trunk segments in the younger larvae and fully developed skeletal muscle with a division of red and white cells in the older larvae. The stem cell marker PAX7, which is present in all developmental stages of teleost fish, is only expressed in the younger dogfish. The results show the necessity of examining the skeletal muscle development in sharks to understand the evolutional changes from cartilaginous fishes to teleosts. |
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| Keywords: | immunohistochemistry myosin PAX7 Western blot cartilaginous fish |
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