Detection of protein-ligand interaction on the membranes using C-terminus biotin-tagged alamethicin |
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Authors: | Zhang Y Futaki Shiroh Kiwada Tatsuto Sugiura Yukio |
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Affiliation: | Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. |
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Abstract: | C-terminal biotin-tagged alamethicin, which has several alpha-aminoisobutyric acid (Aib) residues in its sequence, was synthesized by the preparation of the protected peptide segment using the 2-chlorotrityl resin, followed by conjugation with biotin hydrazide. Suppression of the channel current of the biotin-tagged alamethicin by the addition of streptavidin to the electrolyte was monitorable in real time using the planar lipid-bilayer method. The system was also applicable to the detection of interaction of the biotin-tagged alamethicin with the anti-biotin antibody. |
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