DNA from Aspergillus flavus contains 5-methylcytosine |
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Authors: | Humaira Gowher Kenneth C. Ehrlich Albert Jeltsch |
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Affiliation: | Institut für Biochemie, FB 8, Heinrich-Buff-Ring 58, 3392 Giessen, Germany. |
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Abstract: | DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by using a highly sensitive and specific method for detection of modified bases in genomic DNA comprising high-performance liquid chromatography separation of nucleosides, labeling of the nucleoside with deoxynucleoside kinase and two-dimensional thin-layer chromatography. Our results show that 5-methylcytosine is present in DNA from A. flavus. We estimate the relative amounts of 5-methylcytosine to cytosine to be approximately 1/400. |
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Keywords: | 5-Methylcytosine DNA methylation Fluffy phenotype Fungal development Aspergillus flavus |
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