Cross-helix separation of tropomyosin molecules in acto-tropomyosin as determined by neutron scattering. |
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| Authors: | D B Bivin D B Stone D K Schneider and R A Mendelson |
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| Institution: | Cardiovascular Research Institute, University of California, San Francisco 94143. |
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| Abstract: | The cross-helix separation of Tm molecules in acto-tropomyosin has been determined using neutron scattering. Deuterated Dictyostelium discoideum actin was density matched in a 93% D2O buffer so that effectively only the protonated tropomyosin was "visible" to neutrons. Analysis of the solution scattering pattern in the region of the first oscillation yielded a value for the cross-helix separation of 7.9 +/- 0.3 nm. The implications of this value for the mechanism of the regulation of muscle contraction are discussed in light of recent results by others. |
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