Plasmid transformation of Clostridium perfringens by electroporation methods |
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Authors: | Mary K Phillips-Jones |
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Institution: | Department of Applied Biochemistry and Food Science, University of Nottingham, Loughborough, Leics., U.K. |
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Abstract: | Two electroporation methods were compared and modified to improve the frequencies of transfer of plasmid DNA into Clostridium perfringens. A plasmid shuttle vector, pSB92A2, containing chloramphenicol and ampicillin resistance genes and a clostridial origin of replication isolated from a cryptic C. perfringens plasmid, was constructed and successfully introduced into C. perfringens by both electrotransformation methods. Modifications which improved frequencies by 15-28 fold are described and may improve frequencies sufficiently for some vector/host combinations to consider the future use of more direct cloning strategies for the clostridia. |
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Keywords: | Clostridium perfringens Transformation Plasmid shuttle vector cloning vector |
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