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New classes ofStreptomyces coelicolor A3(2) mutants blocked in undecylprodigiosin (Red) biosynthesis |
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| Authors: | E. A. Coco K. E. Narva J. S. Feitelson |
| Affiliation: | (1) Medical Research Division, American Cyanamid Company, 10965 Pearl River, NY, USA;(2) Present address: Dept. of Molecular Biology, Mycogen Corporation, 5451 Oberlin Drive, 92121 San Diego, CA, USA |
| Abstract: | Summary Fifteen mutants ofStreptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on theS. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes. |
| Keywords: | Recombinant DNA Antibiotic production Genetic organization Directed mutant screen Prodigiosin |
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