Evidence for heme release in layer-by-layer assemblies of myoglobin and polystyrenesulfonate on pyrolitic graphite |
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| Authors: | Matheus T de Groot Maarten Merkx Marc T M Koper |
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| Institution: | (1) Laboratory of Inorganic Chemistry and Catalysis, Schuit Institute of Catalysis, P.O. Box 513, 5600 MB Eindhoven, The Netherlands;(2) Laboratory of Macromolecular and Organic Chemistry, Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands;(3) Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands |
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| Abstract: | Layer-by-layer assemblies of myoglobin and polystyrenesulfonate (PSS) on pyrolitic graphite have been investigated with the
goal of determining the origin of the voltammetric response of these films. From the similar midpoint potential, coverage
and electron transfer behavior compared with those of adsorbed free heme, it was concluded that the observed voltammetric
peak is due to heme adsorbed at the electrode surface. This suggests that the interactions between the pyrolitic graphite
electrode, PSS and myoglobin can result in heme release from the protein followed by heme adsorption on the electrode. |
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| Keywords: | Electrochemistry Heme Electron transfer Voltammetry Denaturant |
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