Goldfish Brain (Na+, K+)-ATPase: Purification of the Catalytic Polypeptide and Production of Specific Antibodies |
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| Authors: | Michal Schwartz George J Siegel Nelson Chen Bernard W Agranoff |
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| Institution: | Departments of Biological Chemistry, Neurology and Mental Health Research Institute, University oj Michigan, Ann Arbor, Michigan 48109, U.S.A. |
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| Abstract: | Abstract: The denatured catalytic polypeptide of (Na+, K+)-ATPase of goldfish brain was purified and identified as the 32P-labeled phosphoprotein. The protein served as immunogen for the preparation of rabbit antisera for immunohistochemical application to goldfish tissue sections, using the peroxidase-antiperoxidase indirect method. Labeling in brain cross-sections appears primarily in fibers of the optic nerve layer of the tectum. In optic nerve cross sections, labeling is restricted to fiber bundles. |
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| Keywords: | Goldfish (Na+ K+)-ATPase Immunocytochemistry Visual system Optic nerve |
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