Influence of poly-A:U, dextran sulfate and yeast RNA on the colony forming ability of bone marrow in irradiated mice]

Poly-A:U, dextran sulfate and yeast RNA were shown to increase the number of endogenous (CFU) in sublethally (525 r.) irradiated mouse spleens seemingly as a result of their mutagenic effect on proliferating CFU. The preparations had no effect on the number of exogenous colonies when injected together with bone marrow syngeneic cells transfer from intact donors. Dextran sulfate led to a 2.7 time increase in the number of endogenous colonies in unevenly irradiated mouse spleens mostly due to the CFU migration from the protected sites of the bone marrow. Poly-A:U and yeast RNA complex was ineffective in such an experiment. It is quite possible that the ability of dextran sulfate to increase the migrational potencies of the stem hematogenic cells served as one of the essential factors in the mechanism of its adjuvant activity.