Effect of mono- and divalent cations on polyp morphogenesis in isolated tentacles of Aurelia aurita(Scyphozoa) |
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Authors: | Andrea Hujer Georgia Elizabeth Lesh-Laurie |
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Affiliation: | (1) Department of Biology, Cleveland State University, 44115 Cleveland, Ohio, USA;(2) Department of Biology, University of Colorado at Denver, Campus Box 137, 80217 Denver, Colorado, USA |
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Abstract: | Tentacles excised from syphistoma polyps of Aurelia aurita undergo rapid regeneration to form whole polyps following exposure to an excess or absence of specific ions. It has been shown that a 12–18 h exposure of isolated tentacles to 58 mM excess of Cs+ results in a rapid firing of nematocysts, followed by an accelerated, synchronous polyp morphogenesis. Absence of Mgt2+ from the culture solution for 4–24 h also led to an accelerated, synchronous polyp regeneration. In either experimental set-up, incubation in 5–10 mM hydroxyurea effectively halted regeneration. Exposure to an excess of Li+ (50–200 mm) or K+ (10–50 mM) caused no firing of nematocysts and a percentage of polyp regeneration only slightly higher than control tentacles. Use of the K+ channel blocker tetraethylammonium (TEA; 100–300 mM) lead to similar levels of regeneration. A Ca2+ or K+-reduced artificial culture solution did not enhance regeneration. Ouabain (1 mM) dampened the Cs+ induced acceleration of polyp morphogenesis, and when given without Cs+, elicited a control level response. |
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Keywords: | Regeneration Polyp Morphogenesis Ions Aurelia |
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