Expression and characterization of the type III polyketide synthase 1,3,6,8-tetrahydroxynaphthalene synthase from<Emphasis Type="Italic"> Streptomyces coelicolor</Emphasis> A3(2) |
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Authors: | Miho?Izumikawa Paul?R?Shipley J?rn?N?Hopke Thomas?O′Hare Longkuan?Xiang Joseph?P?Noel Email author" target="_blank">Bradley?S?MooreEmail author |
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Institution: | (1) College of Pharmacy, University of Arizona, 1703 E. Mabel, Tucson, AZ 85721-0207, USA;(2) Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA;(3) Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA |
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Abstract: | Sequence analysis of the metabolically rich 8.7-Mbp genome of the model actinomycete Streptomyces coelicolor A3(2) revealed three genes encoding predicted type III polyketide synthases (PKSs). We report the inactivation, expression, and characterization of the type III PKS homologous SCO1206 gene product as 1,3,6,8-tetrahydroxynaphthalene synthase (THNS). Incubation of recombinant THNS with malonyl-CoA showed THN production, as demonstrated by UV and HPLC analyses. The Km value for malonyl-CoA and the kcat value for THN synthesis were determined spectrophotometrically to be 3.58±0.85 µM and 0.48±0.03 min–1, respectively. The C-terminal region of S. coelicolor THNS, which is longer than most other bacterial and plant type III PKSs, was shortened by 25 amino acid residues and the resulting mutant was shown to be slightly more active (Km=1.97±0.19 µM, kcat=0.75±0.04 min–1) than the wild-type enzyme. |
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Keywords: | Chalcone synthase Polyketide synthase Tetrahydroxynaphthalene |
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