抗人Clq单克隆抗体轻链可变区基因的克隆及序列测定

 抗人Ｃｌｑ单克隆抗体轻链可变区基因的克隆及序列测定陈克敏，朱锡华（第三军医大学免疫学教研室，重庆６３００３８）目前国内外对基因工程抗体的研究较多，但尚未见补体基因工程的研究报道．一般的方法是经ＲＮＡ反转录合成ｃＤＮＡ，再以ＰＣＲ扩增，克隆Ｉｇ可变区基...

Cloning and Sequencing of the V_k Gene of an Anti-human Cl_q Monoclonal Antibody

Although the PCR technique originally described by Orlandi allows the specific amplification of immunoglobulin variable genes from cDNA of mouse hybridoma cell lines using primers derived from FR_1 and FR_4 consensing sequences, in this experiment. instead of cDNA, the genetic elements encoding the VJL region of a murine monoclonal antibody against human Clq,namely ClqVL was generated from genomic DNA, cloned and sequenced. The results showed that the VJL-sequence of the Clq with 333 nucleotides belongs to mouse V_k V gene family, resulted from V_k-J_(k5) rearrangment. The information involved in Clq V_L is important for the understanding of Clq lunction and of the pathogenetic and immunological aspects of autoimmune disease.