A phosphate-stimulated NAD(P)+-dependent glyceraldehyde-3-phosphate dehydrogenase in Bacillus cereus |
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Authors: | Iddar Abdelghani Serrano Aurelio Soukri Abdelaziz |
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Institution: | Laboratoire de Biochimie, Département de Biologie, Facultés des Sciences A?n-Chock, Université Hassan-II, Km 8 route d'El Jadida, B.P. 5366 Maarif, Casablanca, Morocco. |
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Abstract: | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme of central carbon metabolism, was studied in a Bacillus cereus strain isolated from the phosphate layer from Morocco. Enzymatic assays with cell extracts demonstrated that when grown on Luria-Bertani (LB) medium, B. cereus contains a major NAD+-dependent GAPDH activity and only traces of NADP+-dependent activity, but in cells grown on Pi-supplemented LB medium a strong increase of the NADP+-dependent activity, that became predominant, occurs concurrently with a GAPDH protein increase. Our results show that B. cereus possesses two GAPDH activities, namely NAD+- and NADP+-dependent, catalyzed by two enzymes with distinct coenzyme specificity and different phosphate regulation patterns. The finding of a phosphate-stimulated NADP+-dependent GAPDH in B. cereus indicates that this bacterium can modulate its primary carbon metabolism according to phosphate availability. |
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Keywords: | Glyceraldehyde-3-phosphate dehydrogenase NAD+ NADP+ Phosphate Soil bacterium Bacillus cereus |
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