绞股蓝悬浮细胞的原生质体再生植株

绞股蓝(Gynostemma pentaphyllum (Thumb)Mak．)是葫芦科多年生草本药用植物，现已得到广泛的开发利用，本文首次报道了绞股蓝悬浮细胞的原生质体再生植株。

Protoplast Culture and Plant Regeneration from the Suspension Cells of Gynostemma pentaphyllum (Thumb) Mak.

While the embryogenic callus induced from young stems of G. pentaphyllum on MS medium with Img/L 2,4-D and 0. 2mg/L KT were cultured in MS liquid medium on a rotary shaker, stable embryogenic suspensions were establidshed. A high yield of protoplasts could be released from the suspension cells incubated in a enzyme solution containing 2%cellulase RS, 0. 1% pectolyase Y-23, 0. 55mol/L mannital and CPW salt for 5 hours. These protoplasts were cultured in defferent medium with liquid and nurse culture method. Cell division was observes within 4 days and microcalli were formed within 4 weeks. After proliferating, protoplast-derived callus differentiated into embry-oid on MS solid medium with Img/L KT and 0. 5mg/L IAA. Stems and leaves were formed on MS medium with Img/L 6-BA and 0. 5mg/L IAA. Finally, complet plantlets were obtained on hormon free MS solid medium.