| 酵母菌类金属硫蛋白的分离、纯化及性质鉴定 |
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| 引用本文： | 李明春,李登文,胡国武,侯文强,邢来君. 酵母菌类金属硫蛋白的分离、纯化及性质鉴定[J]. 菌物学报, 2001, 20(2): 214-221 |
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| 作者姓名： | 李明春 李登文 胡国武 侯文强 邢来君 |
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| 作者单位： | 南开大学微生物学系 |
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| 摘 要： | 经过对酵母菌的金属抗性试验和培养条件试验，确定了高产类金属硫蛋白的酿酒酵母菌株Cu-21（Saccharomyces cerevisiae Cu-21）及其最佳培养条件。对该菌株的菌体蛋白进行SephadexG-100、DEAE-52结合的两次柱分离纯化，获得了类金属硫蛋白的三个亚型，蛋白性质鉴定结果表明含三个亚型、分子量小、富含半胱氨酸和金属元素，具有典型的巯基吸收特性。
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| 关 键 词： | 类金属硫蛋白 分离纯化 酿酒酵母 |
| 文章编号： | 1007-3515（2001）02-0214-0221 |
| 修稿时间： | 2000-11-20 |
ISOLATION, PURIFICATION AND CHARACTERIZATION OF METALLOTHIONEIN-LIKE PROTEIN FROM SACCHAROMYCES CEREVISIAE |
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| LI Ming-Chun LI Deng- Wen HU Guo-Wu XING Lai-Jun. ISOLATION, PURIFICATION AND CHARACTERIZATION OF METALLOTHIONEIN-LIKE PROTEIN FROM SACCHAROMYCES CEREVISIAE[J]. Mycosystema, 2001, 20(2): 214-221 |
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| Authors: | LI Ming-Chun LI Deng- Wen HU Guo-Wu XING Lai-Jun |
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| Abstract: | Saccharomyces cerevisiae Cu-21 was selected and its culture conditions were determinated. The proteins were isolated and purified by Sephadex G-100 and subsequent DEAE-52, and three types of Cu-MTs were found. The purified proteins were considered to be MTs-like protein by their characters of low-molecular weight, high cysteine and metal content and special absorbency spectrums. |
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| Keywords: | Metallothionein- like protein Isolate and purify Saccharomyces cerevisiae |
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