SuppressorssuaC 109 andsuaA 101 ofAspergillus nidulans alter the ribosomal phenotypein vitro |
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Authors: | A. Zamir S. S. Martinelli |
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Affiliation: | (1) Department of Biology, Birkbeck College, University of London, Malet Street, WC1E 7HX London |
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Abstract: | A new homologous, cell-free system for protein synthesis has been devised for use with ribosomes and elongation factors fromAspergillus nidulans. Ribosome preparations from strains with either the suaAlO1 orsuaCl09 mutations have a higher misreading ratio (non-cognate:cognate amino acid incorporation) in the presence of hygromycin than controls. They can be classed as fidelity mutants. These results also prove that the mutations must be in genes coding for ribosomal proteins or enzymes which modify ribosomal proteins post-translationally. Alternatively, the genes could code for translation factors. |
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Keywords: | suppressors misreading ribosome Aspergillus nidulans |
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