Conserved TcR β chain usage for a single MHC class II-restricted heat shock protein peptide |
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Authors: | Gail E Hawes Linda Struyk Helen Beacock-Sharp Judy Henwood J S Hill Gaston P J van den Elsen |
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Institution: | (1) Department of Immunohematology and Blood Bank, Leiden University Medical Center, The Netherlands, NL;(2) Department of Medicine, University of Cambridge, Cambridge, UK, GB |
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Abstract: | Previously we have shown that the T-cell response against the HLA-DR3 (17)-restricted heat shock protein (M
r 65 000)-derived peptide amino acids (aa) 3–13 (hsp65 aa 3–13) is recognized by the exclusive usage of the TRBV5 gene as well as a conserved CDR3 region in a tuberculoid leprosy patient. In the present study we analyzed the TcR of T-cell
clones specific for hsp65 aa 3–13 derived from three healthy individuals with a response level similar to that of the leprosy
patient. We show that unlike the tuberculoid leprosy patient, healthy high responders have a diverse T-cell response to hsp65
aa 3–13. However, a striking observation was made: even though high responders have a diverse specific TcR repertoire, TRBV5-expressing clones from two healthy individuals could be isolated that were nearly identical to a dominant clone in the tuberculoid
leprosy patient. In conclusion, the data show that restriction of TcR specific for an antigen correlates with the presence
of that antigen in disease. However, the preferred TcR can also be detected in healthy high responders. A natural infection
in vivo, as with the tuberculoid leprosy patient, may be responsible for the observed trimming and preferential outgrowth
of a certain TcR.
Received: 26 January 1998 / Revised: 17 March 1998 |
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Keywords: | TcR T-cell clone Heat shock protein |
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