Alkaline phosphatase conductometric biosensor for heavy-metal ions determination |
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| Institution: | 1. Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Street, Kiev 03143, Ukraine;2. Laboratoire des sciences de l’environnement, École nationale des Travaux publics de l’État, rue Maurice-Audin, 69518 Vaulx-en-Velin cedex, France;3. Institut de recherches sur la catalyse et l’environnement de Lyon, IRCELYON, UMR-CNRS 5256, université Lyon-1, 2, avenue Albert-Einstein, 69626 Villeurbanne cedex, France;4. Centre sciences des processus industriels et naturels, École nationale supérieure des Mines de St-Étienne, 158, cours Fauriel, 42023 Saint-Étienne cedex 2, France |
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| Abstract: | Alkaline phosphatase conductometric biosensors consisting of interdigitated gold electrodes and enzyme membranes have been used for assessment of heavy-metal ions in water. These analytes act as enzyme inhibitors. Enzyme residual activity has been measured in Tris-nitrate buffer without metal preincubation in the presence of Mg2+ ions as activator. The results indicate that the toxicity of the various metals tested toward immobilized phosphatase is ranged as follows: Cd2+ > Co2+ > Zn2+ > Ni2+ > Pb2+. Detection limits were about 0.5 ppm for Cd2+, 2 ppm for both Zn2+ and Co2+, 5 ppm for Ni2+ and 40 ppm for lead ions. In addition, the responses during 10 h were stable (RSD 4%) and a drift of about 7% per day was observed. The storage stability in buffer solution at 4 °C remained stable for more than one month. |
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