没食子酸对PI3K/AKT基因表达的影响及其对胃癌细胞的抗转移作用

为了调查在蔬菜中大量存在的酚酸(没食子酸(GA),咖啡酸(CA)和原儿茶酸(PCA))对胃腺癌(AGS)细胞转移的抑制作用,本研究在不同浓度的CA、PCA或GA中培养了AGS细胞24 h或48 h来检测AGS细胞活性对NF-κB、IκB、PI3K、AKT和小细胞GTPase表达和对细胞骨架F-肌动蛋白模式的影响。本研究发现,0.01 mmol/L GA诱导的细胞毒性与4.0 mmol/L PCA相同。GA对AGS细胞迁移有明显的抑制作用。AGS细胞的MMP-2/9表达被2.0μmol/L GA所抑制。GA对MMP-2/9的抑制作用有可能包括抑制NF-κB活性。多蛋白参与转移和细胞骨架重组信号通路,包括Ras、Cdc42、Rac1、RhoA、RhoB、PI3K和p38MAPK,也被GA所抑制。此外,细胞骨架F-肌动蛋白的免疫反应检测显示GA处理的显著抑制作用。本研究表明,GA有可能成为一种有效的预防和治疗胃癌转移的药物。

Effects of Gallic Acid on the Expression of PI3K/AKT Gene and Its Anti-metastasis Effect on Gastric Cancer Cells

To investigate the inhibitory effects of phenolic acids(gallic acid(GA),caffeic acid(CA)and procatechic acid(PCA),which are abundant in vegetables,on gastric adenocarcinoma(AGS)cell metastasis,AGS cells were cultured at different concentrations of CA,PCA or GA for 24 h or 48 h to detect the activity of AGS cells,the expression of NF-κB,IκB,PI3 K,AKT,and small-cell GTPase,and the effect on the cytoskeleton F-actin pattern.It was founed that the cytotoxicity induced by 0.01 mmol/L GA was the same as that induced by 4.0 mmol/L PCA.GA significantly inhibited the migration of AGS cells.The expression of MMP-2/9 in AGS cells was inhibited by 2.0 M GA.GA inhibition of MMP-2/9 may include inhibition of NF-κB activity.Polyproteins involved in metastasis and cytoskeletal reprogramming signaling pathways,including Ras,Cdc42,Rac1,Rho A,Rho B,PI3 K,and p38 MAPK,are also inhibited by GA.In addition,detection of the immune response of cytoskeleton F-actin showed significant inhibition of GA treatment.This research indicated that GA might be an effective drug for the prevention and treatment of gastric cancer metastasis.