Landscape and Dynamics of the Transcriptional Regulatory Network During Natural Killer Cell Differentiation |
| |
| Authors: | Kun Li Yang Wu Young Li Qiaoni Yu Zhigang Tian Haiming Wei Kun Qu |
| |
| Affiliation: | Department of Oncology,The First Affiliated Hospital of USTC,Division of Molecular Medicine,Hefei National Laboratory for Physical Sciences at Microscale,School of Basic Medical Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230021,China;Department of Oncology,The First Affiliated Hospital of USTC,Division of Molecular Medicine,Hefei National Laboratory for Physical Sciences at Microscale,School of Basic Medical Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230021,China;CAS Center for Excellence in Molecular Cell Sciences,The CAS Key Laboratory of Innate Immunity and Chronic Disease,University of Science and Technology of China,Hefei 230027,China;Department of Oncology,The First Affiliated Hospital of USTC,Division of Molecular Medicine,Hefei National Laboratory for Physical Sciences at Microscale,School of Basic Medical Sciences,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230021,China;CAS Center for Excellence in Molecular Cell Sciences,The CAS Key Laboratory of Innate Immunity and Chronic Disease,University of Science and Technology of China,Hefei 230027,China;School of Data Science,University of Science and Technology of China,Hefei 230026,China |
| |
| Abstract: | Natural killer (NK) cells are essential in controlling cancer and infection. However, little is known about the dynamics of the transcriptional regulatory machinery during NK cell differentiation. In this study, we applied the assay of transposase accessible chromatin with sequencing (ATAC-seq) technique in a home-developed in vitro NK cell differentiation system. Analysis of ATAC-seq data illustrated two distinct transcription factor (TF) clusters that dynamically regulate NK cell differentiation. Moreover, two TFs from the second cluster, FOS-like 2 (FOSL2) and early growth response 2 (EGR2), were identified as novel essential TFs that control NK cell maturation and function. Knocking down either of these two TFs significantly impacted NK cell differentiation. Finally, we constructed a genome-wide transcriptional regulatory network that provides a better understanding of the regulatory dynamics during NK cell differentiation. |
| |
| Keywords: | NK cell ATAC-seq Programmed differentiation FOSL2 EGR2 Dynamic regulatory network |
| 本文献已被 万方数据 ScienceDirect 等数据库收录！ |
|
