Multipurpose Love acoustic wave immunosensor for bacteria,virus or proteins detection |
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Affiliation: | 1. Laboratoire de l’intégration du matériau au système (IMS), université Bordeaux-1, ENSEIRB, CNRS UMR 5218, 33405 Talence, France;2. Institut des sciences moléculaires (ISM), université Bordeaux-1, CNRS UMR 5255, 33405 Talence, France;3. Laboratoire d’immunologie et parasitologie, université Bordeaux-2–Victor-Segalen, 33076 Bordeaux cedex, France;4. AGUR, 5, rue de la Feuillée, 64100 Bayonne, France |
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Abstract: | A multipurpose Love acoustic wave biosensor is described in this article. As mass loading is one of the main effect involved in acoustic wave sensors, a great range of biomolecules could be detected using such sensors. In this way, the antibody/antigen binding property was used to immobilise the target species. We first compared different coupling agents to link the antibodies sensitive layer to the SiO2 sensor surface. Results showed that GPTS monolayer, allowing covalent attachment of antibodies bioreceptors, is better suited than DTSP and protein G. It permits to obtain a dense, stable and reproducible sensitive layer of antibodies. Then, different biological species with different size and shape like proteins, bacteriophages or bacteria were detected using such sensor. Different models have been chosen to validate the effective detection of a large species range: an anti-mouse antibody has been used to simulate small molecules (< 10 nm) like proteins or toxins, bacteriophage M13 for species lower than 1 μm like virus, and Escherichia coli for bacteria which are typically longer than 1 μm. Each kind of species were successfully quickly detected from few seconds for small proteins to one hour for bacteria, with detection threshold down to 4 ng/mm2 for protein and 106 cfu per milliliter for bacteria. |
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