载氢-纳米氧化铈微泡对小鼠造血系统抗辐射作用的分析

为探讨载氢-纳米氧化铈微泡对小鼠辐射损伤的防护作用。本研究检测载氢-纳米氧化铈微泡的表征,并将60只BALB/c小鼠随机分为正常对照组、照射对照组、载氢-纳米氧化铈微泡组。小鼠经6Gy x射线一次性全身照射(剂量率2 Gy/min)。于照射后3 d和8 d处死小鼠,检测其外周血细胞数、脾脏和胸腺指数、骨髓和脾脏组织病理学变化。结果显示,照射后3 d和8 d,与正常对照组相比,载氢-纳米氧化铈微泡组和照射对照组的白细胞均明显下降,相比照射对照组,载氢-纳米氧化铈微泡组有改善(p<0.05或p<0.01);而载氢-纳米氧化铈微泡组和照射对照组的红细胞数和血红蛋白均略有下降,但差异无统计学意义。与正常对照组相比,微泡组的胸腺指数、脾脏指数均有下降,和照射对照组相比,载氢-纳米氧化铈微泡组的胸腺指数明显改善(p<0.05或p<0.01)。照射后3 d,与正常对照组相比,照射对照组的骨髓细胞较少,存在细胞碎片,载氢-纳米氧化铈微泡组骨髓细胞数量略有减少,存在细胞核松散现象。而照射后8 d,与正常对照组相比,照射对照组的骨髓细胞几乎找不到,载氢-纳米氧化铈微泡组骨髓细胞有一定数量,存在细胞凋亡现象。本研究表明,载氢-纳米氧化铈微泡通过保护造血组织、改善造血功能,对机体起到一定的辐射防护作用。

Analysis of Anti-radiation Effect of Hydrogen-nanocelidium Oxide Microbubbles on Hematopoietic System in Mice

In order to explore protective effect on radiation injury of hydrogen-nanocerium oxide microbubbles in mice,the characterization of hydrogen-nanocerium oxide microbubbles was detected.Then sixty BALB/c mice were randomly divided into normal control group,irradiation control group and microbubble group.Mice received one-time whole body irradiation by 6 Gy X-ray(dose rate:2 Gy/min).Mice were sacrificed on day 3 and day 8 after irradiation.Peripheral blood cell count,spleen and thymus index,bone marrow and spleen histopathological changes were detected.The results showed that three and eight days after irradiation,compared with the normal control group,the white blood cells in the microbubble group and the irradiated control group all decreased significantly.Compared with the irradiated control group,the microbubble group showed improvement(p<0.05 or p<0.01).Erythrocyte and hemoglobin in the microbubble group and the irradiated control group decreased slightly,but the difference was not statistically significant.Compared with the normal control group,the thymus index and spleen index of the microbubble group all decreased slightly.Compared with the irradiated control group,thymus index in the microbubble group was significantly improved(p<0.05 or p<0.01).Three days after irradiation,compared with the normal control group,the irradiated control group had fewer bone marrow cells with cell fragments.The number of bone marrow cells in the hydrogen-nano-ceric oxide microbubble group decreased slightly,and there was loose nucleus.However,eight days after irradiation,compared with the normal control group,there were almost no bone marrow cells in the irradiated control group.There were a certain number of bone marrow cells in the hydrogen-nanoceric oxide microbubble group,which was apoptosis.This study shows that hydrogen-carrying cerium oxide microbubbles play a role in radiation protection by protecting hematopoietic tissue and improving hematopoietic function.