球孢白僵菌中聚酮合酶基因多样性分析

本研究利用基因挖掘技术从球孢白僵菌(Beauveria bassiana)基因组中获得聚酮合酶(PKS)基因,并对这些基因序列进行生物信息学分析预测其功能,同时检测这些基因在不同培养基培养条件下的表达情况。结果显示:球孢白僵菌中含有13个PKS(Bdass1~13)基因,结构域分析显示球孢白僵菌中有还原型PKS 8个,非还原型PKS 2个,杂合型NRPS/PKS 3个;聚类分析显示Bdass8参与卵孢白僵菌素生物合成,Bdass5可能参与洛伐他汀九酮体的生物合成、Bdass4可能参与伏马菌素的生物合成、Bdass11可能参与phenolthiocerol的生物合成;非还原型PKS中Bdass7和Bdass10可能参与分生孢子色素的合成,Bdass1、Bdass2、Bdass3、Bdass6、Bdass9、Bdass12、Bdass13分别与其他未知聚酮合酶形成独立的分支;不同的PKS基因在不同培养基培养条件下其表达情况差异非常显著,如Bdass8、Bdass10和Bdass11在5种培养基上均强烈表达,Bdass4、Bdass6在5种培养基上微弱表达,Bdass1、Bdass5、Bdass11、Bdass12、Bdass13仅在几种培养基上表达,Bdass2仅在INO培养上微弱表达,Bdass3、Bdass7在5种培养基上均不表达。该研究为球孢白僵菌中PKS基因的功能鉴定奠定基础。

The Diversity Analysis of Polyketides Synthase(PKS)Gene in Beauveria bassiana

The present text has got polyketides synthase(PKS)genes from the genome of Beauveria bassiana with the method of genome-mining and analyzed these genes to predict their function by the bioinformatic analysis,simultaneously tested the condition of these gene expression in the different medium.The results showed that there were 13 PKS gene in the genome of B.bassiana,including 8 reducing PKS(R-PKS),2 non-reducing PKS(NR-PKS)and 3 hybrid NRPS/PKS;the phylogenetic tree revealed that Bdass8 involved in the biosynthesis of tenellin,Bdass5 might involve the biosynthesis of lovastatin ninoketone,Bdass4 could involve the biosynthesis of fumonisin,and Bdass11 might involve the biosynthesis of phenolthiocerol among the reducing PKS;and among the nonreducing PKS Bdass7 and Bdass10 could involve the biosynthesis of conidial yellow pigment;additionally,Bdass1,Bdass2,Bdass3,Bdass6,Bdass9,Bdass12,Bdass13 clustered with some unkown product that they involved together,respectively;the gene expression had significant difference when the different PKS genes were cultivating in the different medium,for example,Bdass8,Bdass10 and Bdass11 was strongly expressed cultivating in the 5 medium,Bdass4,Bdass6 was slightly expressed in all medium,Bdass1,Bdass5,Bdass11,Bdass12 and Bdass13 was expressed in some special medim,Bdass2 was only expressed in the INO,Bdass3 and Bdass7 were never expressed in all medium.The study took some basis for the PKS gene function of B.bassiana.