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An interspecific somatic hybrid between Actinidia chinensis and Actinidia kolomikta and its chilling tolerance
Authors:Zun-an Xiao   Lin-chen Wan  Bi-wen Han
Affiliation:(1) College of Life Sciences, Beijing Normal University, Beijing, 100875, China;(2) College of Biology, China Agricultural University, Beijing, 100094, China
Abstract:Protoplasts isolated from cotyledon-derived callus of Actinidia chinensisPlanch. var. chinensis (2N=2x=58) were fused with mesophyll protoplasts of Actiniadia kolomikta(Maxim. et Rupr.) Maxim (2N=2x=58) using a PEG method. Plantlets were regenerated from the fusion product clone 11. RAPD analyses, chromosome numbers of root tip cells and fluorescence peak position of leaf nuclei confirmed that clone 11 was an interspecific somatic hybrid (2N=4x=116) between A. chinensis and A. kolomikta. The chilling tolerance of the somatic hybrid was tested with in vitro leaves at low temperatures. Based on data of leaf thickness, electroconductivity, proline levels, malondialdehyde content and activity of superoxide dismutase, dendrogram cluster analysis suggested that the interspecific somatic hybrid was similar to A. kolomikta, and might have a higher capacity of cold resistance than A. chinensis.
Keywords:cold hardiness  electroconductivity  flow cytometry  kiwifruit  malondialdehyde  proline  protoplast fusion  RAPD markers  superoxide dismutase
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