High-pressure liquid chromatography of carcinogen-nucleoside conjugates: Separation of 7,12-dimethylbenzanthracene derivatives |
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| Affiliation: | 1. Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032 USA;2. Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032 USA;3. Ben May Laboratory for Cancer Research, University of Chicago, Chicago, Illinois 60637 USA;1. Guangdong Provincial Key Laboratory of Food Quality and Safety/Guangdong Laboratory of Lingnan Modern Agriculture, South China Agricultural University, Guangzhou, 510642, China;2. Food Science and Technology Program, BNU-HKBU United International College, Zhuhai, Guangdong 519087, China;1. Department of Chemistry & Chemical Biology, Northeastern University, Boston, MA 02115, United States;2. Greenlight Biosciences, Medford, MA, 02155, United States |
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| Abstract: | Conditions for obtaining rapid, high resolution separations of nucleic acid derivatives of 7,12-dimethylbenzanthracene using reverse phase high-pressure liquid chromatography are described. Direct reaction of dimethylbenzanthracene-5,6-oxide with poly(G) or poly(A) yields four and two major dimethylbenzanthracene-nucleoside conjugates, respectively. Incubation of [3H]dimethylbenzanthracene with poly(G) in a microsomal system leads to the formation of numerous guanosine derivatives, most of which differ from the 5,6-oxide products. The high sensitivity and resolution obtained in these studies demonstrate that high-pressure liquid chromatography will be a valuable technique for identification and characterization of other carcinogen-nucleic acid complexes. |
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