Alkaline phosphatase in mitochondria |
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| Affiliation: | 1. Department of Cellular Pathology, Imperial Cancer Research Fund, Lincoln''s Inn Fields, London, WC2A 3PX, UK;7. MRC Human Biochemical Genetics Unit, The Galton Laboratory, University College London, 4 Stephenson Way, London, NW1 2HE, UK;1. Center of the Spinal Surgery, Nipponbashi Hospital, Osaka-shi, Osaka, Japan;2. Department of Neurosurgery, Kyoto University, Kyoto-shi, Kyoto, Japan;3. Sakuragaoka Pathological Diagnostic Clinic, Kusatu-shi, Shiga, Japan;1. University of Dresden, Department of Neurology, Fetscherstrasse 74, 01307 Dresden, Germany;2. GWD Consult, Contract Research, Mozartstrasse 2–4, 63165 Mühlheim am Main, Germany;3. TEVA Pharma GmbH, Charlottenstraße 59, 10117 Berlin, Germany;1. Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas – UFPel, Pelotas, RS, Brazil;2. Núcleo de Tecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas – UFPel, Pelotas, RS, Brazil;3. Centro Nacional Gado de Corte, Empresa Brasileira de Pesquisa Agropecuária – Embrapa, Campo Grande, MS, Brazil;1. Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan;2. Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan;3. Division of Biochemical Engineering, Radioisotope Research Center, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan;1. Laboratory of Bone Biology, Department of BioMolecular Sciences, School of Pharmaceutical Sciences Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil;2. Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil;3. Center for Research in Inflammatory Diseases, CRID, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil |
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| Abstract: | In electron microscope cytochemical studies alkaline phosphatase activity was present in the mitochondria of all liver cells and associated with the plasma membrane of the cells of bile canaliculi. The mitochondrial activity was partially inhibited by L-phenylalanine and Levamisole but the plasma membrane associated activity was completely inhibited by Levamisole. Biochemical assays have shown that a significant amount of the total mouse liver alkaline phosphatase activity was present in the mitochondrial fraction. Starch gel electrophoresis showed that this mitochondrial alkaline phosphatase had a characteristic isoenzyme pattern, consisting of 3 distinct bands which were not retarded by neuraminidase treatment. The enzyme in the mitochondria-free supernatant showed one wide band which was retarded by neuraminidase. |
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