|
|||||
|
|
Flow-injection analysis of catecholamine secretion from bovine adrenal medulla cells on microbeads |
|
| Authors: | M Herrera L S Kao D J Curran E W Westhead |
| Affiliation: | 1. Department of Biochemistry, University of Massachusetts, Amherst, Massachusetts 01003 USA;2. Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003 USA;1. Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo 780, Av. E. Garzón, Uruguay;2. Departamento de Morfología y Desarrollo, Facultad de Veterinaria, Universidad de la República, Montevideo 1620, A. Lasplaces, Uruguay;1. Norwegian Institute of Bioeconomy Research, Ås, Norway;2. National Institute of Occupational Health, Oslo, Norway;1. College of Life Sciences, Xinyang Normal University, Xinyang 464000, China;2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China;3. Henan Institute of Animal Health Supervision, Zhengzhou 450008, China;4. Henan Center of Animal Products Quality Monitoring and Test, Zhengzhou 450008, China |
| Abstract: | Bovine adrenal medullary cells have been cultured on microbeads which are placed in a low-volume flow system for measurements of stimulation-response parameters. Electronically controlled stream switching allows stimulation of cells with pulse lengths from 1 s to many minutes; pulses may be repeated indefinitely. Catecholamines secreted are detected by an electrochemical detector downstream from the cells. This flow-injection analysis technique provides a new level of sensitivity and precision for measurement of kinetic parameters of secretion. A manual injection valve allows stimulation by higher levels of stimulant in the presence of constant low levels of stimulant. Such experiments show interesting differences between the effects of K+ and acetylcholine on cells partially desensitized to acetylcholine. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |