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Sterol composition of normal and habituated sugarbeet callus (Beta vulgaris L.Altissima) |
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| Authors: | Paulette Schmitt Thomas Gaspar Daniel Hagège |
| Institution: | (1) Enzymologie Cellulaire et Moléculaire, Institut de Botanique, IBMP, 28 rue Goethe, F-67083 Strasbourg, France;(2) Hormonologie fondamentale et appliquée, Institut de Botanique B22, Université de Liège, Sart Tilman, B-4000 Liège, Belgique;(3) Biochimie Végétale, UFR Sciences et Techniques, BP 452, F-29275 Brest cedex, France |
| Abstract: | Summary A detailed qualitative and quantitative analysis of the sterol content of normal (auxin and cytokinin requiring) and habituated (auxin and cytokinin independent) sugarbeet callus (Beta vulgaris L.altissima) was made using mass spectrometry with gas chromatography. The total sterol content of the two lines did not differ significantly. Δ7-Sterols were the most important class of sterols in the two sugarbeet callus lines, as in allChenopodiaceae. Elevated levels of Δ8-sterols were found in the habituated callus. These sterols are considered to be badly integrated in the membrane of eucaryotic cells. A partial blocking of Δ8-Δ7-isomerase is hypothesized in the habituated cell line. |
| Keywords: | callus habituation sterols Δ 8-Δ 7 sterol isomerase Beta vulgaris |
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