| 微生物学实验模块“蛋白酶产生菌的筛选、培养和选育”的教改研究与实践 |
| |
| 引用本文： | 张茵,徐旭士,何伟,袁生.微生物学实验模块“蛋白酶产生菌的筛选、培养和选育”的教改研究与实践[J].微生物学通报,2010,37(11):1698-1700. |
| |
| 作者姓名： | 张茵 徐旭士 何伟 袁生 |
| |
| 作者单位： | 南京师范大学生命科学学院,江苏,南京,210046 |
| |
| 基金项目： | 江苏省高等教育教改立项研究课题项目(No. 200970) |
| |
| 摘 要： | 围绕"蛋白酶产生菌的筛选、培养和选育"主线,设计了一个由5个相互关联、前后衔接、形成一体的实验组成的综合性研究型实验模块,并进行教学实践。它们是:培养基的配制、分装与灭菌;产蛋白酶细菌的分离与纯化;产蛋白酶细菌的培养发酵与条件优化;产蛋白酶细菌的鉴定;产蛋白酶细菌的紫外诱变育种。
|
| 关 键 词： | 微生物学实验 实验模块 蛋白酶产生菌 |
A Experiment Module of Isolation, Cultivation and Breeding of Protease-producing Bacteria for Microbiological Experiment Teaching |
| |
| ZHANG Yin,XU Xu-Shi,HE Wei and YUAN Sheng.A Experiment Module of Isolation, Cultivation and Breeding of Protease-producing Bacteria for Microbiological Experiment Teaching[J].Microbiology,2010,37(11):1698-1700. |
| |
| Authors: | ZHANG Yin XU Xu-Shi HE Wei and YUAN Sheng |
| |
| Institution: | College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210046, China;College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210046, China;College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210046, China;College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu 210046, China |
| |
| Abstract: | We integrated a group of related experiments focusing on isolation, cultivation and mutation breeding of protease-producing bacteria into an experiment module and applied it in microbiological experiment teaching. The experiment module included preparation and sterilization of media, isolation of protease-producing bacteria, optimization of cultivation conditions, characterization of the strain, mutation breeding of the strain by UV exposure. |
| |
| Keywords: | microbiological experiment experiment module protease-producing bacteria |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
|
