Transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae. |
| |
| Authors: | K Takegawa S Yamaguchi A Kondo H Iwamoto M Nakoshi I Kato S Iwahara |
| |
| Institution: | Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan. |
| |
| Abstract: | Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae has transglycosylation activity. Treatment of (Man)6(GlcNAc)2Asn with the enzyme in the presence of free N-acetylglucosamine gave a mixture of (Man)6GlcNAc and (Man)6GlcNAc beta 1----4GlcNAc mixture. N-Acetylglucosamine at the reducing end of the latter sugar chain was found by HPLC of the carbohydrate composition and of an exoglycosidase digest of the pyridylamino derivative of the reducing-end residue, and by 400 MHz 1H-NMR spectroscopy. |
| |
| Keywords: | |
|
|
