An adaptor strategy to subclone entire cDNA libraries as single insert recombinants |
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Authors: | E C B?ttger |
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Institution: | Medizinische Hochschule Hannover, Institut für Medizinische, Mikrobiologie, FRG. |
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Abstract: | The current methods for subcloning entire cDNA libraries usually result in a significant portion of recombinants containing multiple inserts, since in most instances the inserts derived from the library to be subcloned are released as a bulk of self-ligatable DNA fragments. By use of an adaptor strategy, a method is presented to confer noncompatible ends to primarily self-ligatable inserts, resulting in efficient subcloning of entire libraries as single insert recombinants. |
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