Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated molecule |
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| Authors: | C A Whitlock G F Tidmarsh C Muller-Sieburg I L Weissman |
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| Institution: | 1. EMLine.org, Mendoza, Argentina;2. Department of Radiation Medicine, Oregon Health & Science University, Portland, OR;1. Division of Immunology, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia;2. The Finsen Laboratory, Bioinformatics Centre, Department of Biology, and Biotech Research and Innovation Center (BRIC), University of Copenhagen, 1165 Copenhagen, Denmark;3. Department of Hematology and Oncology, University Hospital, Otto-von-Guericke University, 39120 Magdeburg, Germany;4. Children’s Cancer Institute Australia, Lowy Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia;5. Department of Haematology, Royal Brisbane and Women’s Hospital, Brisbane, QLD 4006, Australia;6. Queensland Children’s Medical Research Institute, Brisbane, QLD 4029, Australia;7. University of Queensland, Brisbane, QLD 4072, Australia;8. Department of Hematology and Oncology, University Hospital Ulm, 89081 Ulm, Germany;9. Department of Pediatrics, Memorial Sloan Kettering Leukemia Center, New York, NY 10065, USA;10. Division of Hematology and Oncology, Dana-Farber Cancer Institute, Harvard Medical School and Harvard Stem Cell Institute, Cambridge, MA 02138, USA;1. Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada;2. Advanced Molecular Diagnostic Laboratory, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada;3. Clinical Laboratory Genetics, Laboratory Medicine Program, University Health Network, Toronto, Ontario, Canada;4. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada;5. Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada;1. Noor Research Center for Ophthalmic Epidemiology, Noor Eye Hospital, Tehran, Iran;2. Rio de Janeiro Corneal Tomography and Biomechanics Study Group, Rio de Janeiro, RJ, Brazil;3. Federal University of the State of Rio de Janeiro (UNIRIO), Brazil;4. St Paul’s Eye Unit, Royal Liverpool and Broadgreen University Hospital, Liverpool, United Kingdom;5. Humanitas University, Department of Biomedical Sciences, Pieve Emanuele Milan, Italy;6. Humanitas Clinical and Research, Rozzano, Italy;7. Department of Ophthalmology & Visual Science, Department of Biomedical Engineering, The Ohio State University, Columbus, OH, USA;8. Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran;9. Noor Ophthalmology Research Center, Noor Eye Hospital, Tehran, Iran |
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| Abstract: | Bone marrow stromal cell lines have been isolated that directly support B lymphopoiesis in vitro. Single B-lineage precursors proliferate and differentiate on certain of these stromal cell lines to establish long-term B-lineage cultures. These lymphopoietic stromal cells produce novel soluble factors that support proliferation of in vitro established pre-B cell populations. Lymphoid populations established on lymphopoietic stromal cell lines lack surface Ig-bearing cells, but give rise to surface Ig+ cells when transferred to mixed bone marrow feeder layers. Several stromal lines expressed a B-lineage neoplasia marker detected by the monoclonal antibody MAb6C3. Remarkably, only the 6C3Aghi stromal lines supported long-term proliferation of B-lineage cells. We propose that the 6C3 antigen-bearing molecule may play a role in stromal cell-dependent, pre-B cell proliferation, as well as in neoplastic proliferation of pre-B leukemias. |
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