Microprojectile bombardment-mediated transformation of Lilium longiflorum |
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Authors: | A. A. Watad D.-J. Yun T. Matsumoto X. Niu Y. Wu A. K. Kononowicz R. A. Bressan P. M. Hasegawa |
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Affiliation: | (1) Department of Ornamental Horticulture, The Volcani Center, Agricultural Research Organization, P. O. Box 6, Bet-Dagan 50250, Israel, IL;(2) Department of Horticulture, Purdue University, West Lafayette, IN 47907, USA, US |
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Abstract: | We have obtained transgenic lily (Lilium longiflorum) plants after microprojectile bombardment, using the Biolistics PDS 1000/He system, of morphogenic calli derived from bulblet scales, followed by bialaphos selection. Parameters which gave the highest transient uidA expression were used: a bombardment pressure of 1100 psi, a target distance of 6 cm and a 48-h preculture on medium with 3% sucrose. A total of 1800 morphogenic calli were co-bombarded with plasmids containing either the uidA reporter or PAT selectable marker genes. After bombardment, the calli were exposed to 2 mg/l bialaphos. Only 72 of the shoot-forming calli (4%) survived. The 72 shoot clusters produced 342 shoots on elongation medium containing 0.5 mg/l bialaphos. Only 55 plantlets survived subsequent exposure to 2.0 mg/l bialaphos. PCR analysis indicated that 19 of these plantlets contained the PAT transgene. Southern analysis of 3 of the plants indicated that all contained the PAT gene. Received: 21 March 1997 / Revision received: 8 July 1997 / Accepted: 7 August 1997 |
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Keywords: | Lily Transformation Bialaphos Adventitious shoot regeneration |
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