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The effect of cultural conditions on the accumulation of formononetin by suspension cultures of Glycyrrhiza glabra
Authors:C. Arias-Castro  A. H. Seragg  M. A. Rodriguez-Mendiola
Affiliation:(1) Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, S10 2TN Sheffield, UK;(2) Department of Biological Sciences, The University of the West of England, BS16 1QY Bristol, UK
Abstract:Secondary product accumulation by suspension cultures of Glycyrrhiza glabra has been studied. The cultures did not accumulate either glycyrrhizin of glycyrrhetic acid but did produce the isoflavonoid formononetin. The effect of initial pH, sucrose concentrations, medium composition, auxin and cytokinins on formononetin accumulation was studied. Initially formononetin accumulation was non-growth related but this changed to growth associated when the medium was modified.Abbreviations 2,4-d dichlorophenoxyacetic acid - NAA napthalenacetic acid - IAA indoleacetic acid - GA glycyrrhizic acid - beta-GA beta-glycyrrhetic acid
Keywords:formononetin accumulation  Glycyrrhiza glabra  suspension cultures
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