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Enhanced integration of large DNA into E. coli chromosome by CRISPR/Cas9
Authors:Mu‐En Chung  I‐Hsin Yeh  Li‐Yu Sung  Meng‐Ying Wu  Yun‐Peng Chao  I‐Son Ng  Yu‐Chen Hu
Affiliation:1. Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan;2. Department of Chemical Engineering, Feng Chia University, Taichung, Taiwan;3. Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan;4. 886‐3‐571‐8245886‐3‐571‐5408
Abstract:
Keywords:CRISPR/Cas9  double strand break  E. coli  genome editing  integration  metabolic engineering
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