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Translation system engineering in Escherichia coli enhances non‐canonical amino acid incorporation into proteins
Authors:Rui Gan  Jessica G. Perez  Erik D. Carlson  Ioanna Ntai  Farren J. Isaacs  Neil L. Kelleher  Michael C. Jewett
Affiliation:1. Department of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois;2. Department of Chemistry, Northwestern University, Evanston, Illinois;3. Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois;4. Systems Biology Institute, Yale University, West Haven, Connecticut;5. Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut;6. Department of Molecular Biosciences, Northwestern University, Evanston, Illinois;7. 84746750078474913728;8. Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, Illinois;9. Northwestern Institute on Complex Systems, Northwestern University, Evanston, Illinois;10. Simpson Querry Institute, Northwestern University, Evanston, Illinois
Abstract:
Keywords:noncanonical amino acid  orthogonal translation system  aminoacyl‐tRNA synthetase  elongation factor Tu  synthetic biology  genomically recoded organism  directed evolution
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