Fluorescein Diacetate Hydrolysis as a Measure of Fungal Biomass in Soil |
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Authors: | María L Gaspar Marta N Cabello Ricardo Pollero Miguel A Aon |
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Institution: | (1) Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP) (UNLP-CONICET) Calles 60 y 120, 1900 - La Plata, Argentina, AR;(2) Instituto Spegazzini, Calle 53, Nro. 477, Universidad Nacional de La Plata, 1900 - La Plata, Argentina, AR;(3) Instituto Tecnológico de Chascomús/Instituto de Investigaciones Biotecnológicas (CONICET), C.C. 164, 7130-Chascomús, Buenos Aires, Argentina, AR |
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Abstract: | The fatty acid methyl esters of lipids extracted from an agricultural soil in the preharvest period of soybean or middle growth
cycle from wheat were characterized and quantified by gas-liquid chromatography. The fatty acids 18:2ω6 and 16:1ω5 were used
as markers of saprotrophic and arbuscular mycorrhizal fungi. In parallel, biomass estimation through plate counts in selective
media for cellulolytic and saprotrophic fungi was also performed all throughout a soybean crop or middle growth cycle of wheat.
As an enzymatic method, the fluorescein diacetate (FDA) hydrolytic activity of the samples was determined. Owing to the high
relationship exhibited by FDA hydrolysis with organic carbon and total nitrogen content of soil, the enzymatic activity was
correlated with the microbial biomass estimated through marker lipids or plate counts. The results obtained point out that
FDA hydrolysis may be used as a rapid, cheap, and reliable estimator of fungal biomass.
Received: 3 August 2000 / Accepted: 13 October 2000 |
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